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KMID : 0880220090470050572
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Journal of Microbiology
2009 Volume.47 No. 5 p.572 ~ p.581
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Ligand-Receptor Recognition for Activation of Quorum Sensing in Staphylococcus aureus
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Chen Li-Chun
Tsou Li-Tse
Chen Feng-Jui
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Abstract
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The accessory gene regulator (agr) locus controls many of the virulence toxins involved in Staphylococcus aureus pathogenesis, and can be divided into four specificity groups. AgrC is the only group-specific receptor to mediate both intra-group activation and inter-group inhibition. We studied the ligand-receptor recognition of the agr system in depth by using a luciferase reporter system to identify the key residues responsible for AgrC activation in two closely related agr groups, AgrC-I, and AgrC-IV. Fusion PCR and site-directed mutagenesis were used to screen for functional residues of AgrC. Our data suggest that for AgrC-IV activation, residue 101 is critical for activating the receptor. In contrast, the key residues for the activation of AgrC-I are located at residues 49~59, 107, and 116. However, three residue changes, T101A, V107S, I116S, are sufficient to convert the AIP recognizing specificity from AgrC-IV to AgrC-I.
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KEYWORD
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histidine protein kinases, ligands, protein structure, quorum sensing, transmembrane receptors, two-component system
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